The unique Krystal 2000 black & white plate from Porvair Sciences Ltd is purpose designed to provide optimised performance with Chemiluminescence assays.
The Krystal 2000 black and white plate's novel individual white wells, in an opaque black polystyrene matrix design, is achieved using a patented "two-shot" manufacturing process. The special design of the
plate totally eliminates the well-to-well optical crosstalk inherent with plain white plates, reducing false positives and leading to unmatched accuracy, sensitivity and repeatability of photometric readings. Very high signal-to-noise ratio and low detection limits can be achieved in chemiluminescence applications including immunoassays, enzyme assays, reporter gene assays, ATP determinations, RNA and DNA quantitation, cytotoxicity measurements, mycoplasma detection, beta-galactosidase assays and cell proliferation measurements.
The problem of a bright well "masking" weaker chemiluminescence signals from adjacent wells is well known. With standard white assay plates light is often transmitted through their rather thin walls. With very low intensity signals characterising chemiluminescence assays, it is important to eliminate this well-to-well crosstalk to achieve the very best detection limits. While modern multi-mode plate readers provide
very good sensitivity for chemiluminescence readings, none of them can accommodate extremely bright wells adjacent to very dim wells in white plates without reporting artificially high readings. In addition whilst these units all have sufficient dynamic range to cope with the variations in intensity, by virtue of their very sensitivity, they will detect optical cross talk through the plate walls.
Designed to conform to the standard SBS/ANSI format the 96-well Krystal 2000 black and white plate is fully compatible with all commercially available top reading luminometers, multi mode readers, robotic sample processors and automated liquid handling systems.
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