Single medium supports both 2D and 3D pluripotent stem cell culture and growth

Amsbio has launched StemFit™ Basic05, an animal origin-free culture medium designed to support scalable manufacturing of human pluripotent stem cells (hPSCs) across both two-dimensional (2D) adherent and three-dimensional (3D) suspension culture.

Because the same medium can be used throughout cell expansion and process scale-up, laboratories moving cells between culture formats can avoid the redevelopment and optimisation of conditions that media changes traditionally require.

Stem cells cultured in StemFit™ Basic05 retain their capacity to differentiate. Amsbio has confirmed that differentiation can proceed directly from 3D aggregates.

High cell viability and uniform spheroid formation are important for laboratories implementing stable, scalable suspension culture. Across multiple human induced pluripotent stem cell (iPSC) lines, StemFit™ Basic05 has demonstrated consistent cell viability, uniform spheroid formation and maintenance of a normal karyotype, supporting its use in process development and reducing the complexity involved.

The medium also supports flexible feeding schedules, with comparable cell expansion recorded even when weekend feeding was omitted.

Find out more: https://www.amsbio.com/stemfit-stem-cell-culture/

Contact Amsbio: +31-72-8080244 / +44-1235-828200 / +1-617-945-5033 /

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