Increased sample purity with high resolution flash cartridges

The demand for fast and easy purification techniques is increasing. Flash Chromatography can be utilised to achieve quick preparative separations - however, increased resolving power is often desired to purify target compounds from closely eluting contaminants. To satisfy the need for flash cartridges with better resolution and higher loading capacity, high-resolution flash cartridges have been developed such as GraceResolv™ from Grace Davison Discovery Sciences. The silica packed in these newer cartridges has been specifically designed to increase cartridge efficiency (plates/meter) and thereby increase resolution of closely eluting compounds. This results in an increase in the target compound recovered at the desired purity level. The increase in resolution also enables a significant increase in loading capacity, over two times greater in some cases, versus traditional flash cartridges. By purifying greater amounts on a given cartridge size, users can reduce purification costs and increase productivity. In order to demonstrate the advantages of higher resolution flash cartridges we compared GraceResolv™ cartridges to standard 50?m flash chromatography cartridges. Experiments were designed to test sample resolution, loading capacity, recovery, and throughput.