Ready-to-use DUB assay kits for targeted enzyme analysis

AMSBIO has introduced a new series of Deubiquitinating (DUB) enzyme assay kits that streamline the analysis of DUB activity in both purified systems and cell-based models. Supplied in a convenient 96-well plate format, these ready-to-use kits eliminate the need for substrate preparation - making them ideal for routine use in high-throughput screening and academic research.

Deubiquitinating enzymes are central regulators of the ubiquitin-proteasome system, controlling protein stability and function in key cellular pathways such as autophagy, DNA repair, immune signalling, and cell proliferation. With around 100 known DUBs involved in both normal physiology and disease mechanisms - including cancer, neurodegeneration, and inflammation - they represent promising targets for therapeutic intervention.

Each AMSBIO DUB assay kit uses a ubiquitin-based substrate labelled with a fluorophore, enabling sensitive and specific detection of enzyme activity via a microplate reader (excitation: 485 nm, emission: 535 nm). Kits are available for a variety of disease-relevant enzymes, including BAP1, USP7, and UCHL1, supporting precise profiling and inhibitor screening efforts.

Designed for both kinetic and endpoint analysis, these assay kits require minimal setup and deliver robust, reproducible results. Researchers in drug discovery, molecular biology, and cellular signalling can now accelerate their workflows while maintaining high data quality.

Applications include studies in tumour suppression, neurodegenerative disease mechanisms, immune response regulation, and more.

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