Kits
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Invitrogen TrueTag Donor DNA Kits from Thermo Fisher Scientific simplify and accelerate cell line engineering with CRISPR-Cas9 or TALEN technology. Researchers can now enrich tagged cells to obtain up to 100% of edited cells, enabling them to skip the time-consuming clonal isolation step and save up to four months on their genome editing experiments, when compared to traditional gene tagging workflows. These off-the-shelf kits provide templates and clean-up columns to generate donor DNA with a one-step PCR workflow which eliminates the need for cloning.
The expanded portfolio of TrueTag Donor DNA Kits supports four types of locus-specific knock-in templates: Fluorescent tagging kits; Epitope tagging kits; Stem cell fluorescent reporter tagging kits; Knockout enrichment kits.
TrueTag Donor DNA Kits are offered for fluorescent and epitope tags including GFP, RFP, BFP, Myc, HA, 6xHis, YFP and DDK.
Applications include genome editing experiments including creating cell lines for disease model studies and reporter assays, tagging genes for localisation and isolation studies and enriching cells with functional gene knockout.
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ILM Guide 2026/27