Ribosomal RNA Removal Kits provide Quick and Easy Protocol in Under One Hour

Arraystar’s cutting-edge technologies focus on regulatory non-coding RNAs – Long non-coding RNAs (LncRNAs) and LncRNA pathways, microRNAs and their target mRNAs, circular RNAs, piwi-interacting RNAs (piRNAs) and more. These non-coding RNAs have shown tissue and development-specific expression patterns along with diverse cellular functions. They are attracting intense research interest with the possibility that they could offer new disease regulators, novel biomarkers and therapeutic targets.  But before you can get at the RNAs of interest, there are a lot of RNAs to remove.

The Arraystar rRNA Removal Kits efficiently remove rRNA from samples, while providing high yields of mRNAs and noncoding regulatory RNAs, such as LncRNAs, microRNAs, small interfering RNAs (siRNAs) and small nucleolar RNAs (snoRNAs), for many species. Arraystar rRNA Removal Kits are available in the UK and most of Europe through Cambio.

The Arraystar rRNA Removal Kit Standard (Human/Mouse/Rat for intact RNA only) removes rRNA from intact RNA samples, while the Arraystar rRNA Removal Kit, Super (Human/Mouse/Rat, for degraded and intact RNA) removes rRNA from intact or partially degraded samples.

In contrast to poly(A)- enrichment, rRNA depletion permits recovery of poly(A)- mRNAs and regulatory RNAs, which play critical roles in diverse biological processes and have been associated with human diseases. The method is based on hybridisation of specific oligonucleotide probes that are designed to target the 4 major eukaryotic ribosomal RNAs (18S, 28S, 5S and 5.8S), as well as mitochondrial rRNA (12S, 16S), with minimal cross-reactivity to other non-rRNA molecules.

The Arraystar rRNA Removal Kit, Super (Human/Mouse/Rat, for degraded and intact RNA) uses multiple short oligonucleotides for each rRNA to ensure that, even in the presence of degraded target rRNA, the rRNA will be completely removed from the sample. The Super kit is suitable for formalin-fixed, paraffin embedded (FFPE) samples.

With both these kits, a quick and easy protocol can be performed in less than one hour, yielding RNA sufficiently depleted of rRNA, especially suited for NGS applications.

Arraystar also offers rRNA removal kits for Gram negative and Gram positive bacteria, alongside an mRNA purification kit.