CRISPR/Cas9 Knockout Cell Lines and Lysates

Amsbio has established a comprehensive portfolio of knock-out cell lines and lysates generated with CRISPR/Cas9 technology.

Using CRISPR/Cas9 gene editing technology for the generation of knock-out cell lines and cell lysates ensures the development of useful tools to validate antibody specificity and elucidate gene function. Knock-out cell lines and cell lysates are widely agreed to be the best negative controls to quickly and confidently prove an antibody’s specificity for a target of interest and ensure reproducibility.

Generated in the most widely used HEK293T, HeLa, and A549 cell lines, Amsbio gene edited homozygous knock-out cell lines work in the cells you know and trust. Consequently, you are no longer limited to highly specialised and altered backgrounds. Amsbio offers only 100% knock-out clonal lines meaning you don't have to work with pools of cells containing only a percentage of knockout cells.

Knock-out cell lysates from Amsbio are made from double knock-out cell lines and provide the perfect negative control for Western Blots to validate the specificity of antibodies or be used in functional assays. Each Amsbio knock-out cell lysate product comes with a cell-line matched wild type control.