Optimised E.coli Strain Expresses Insoluble Proteins In Soluble Form

Amsbio has announced Solu BL21™ Competent E. coli, a significantly improved BL21 host strain for soluble mammalian protein expression. Using a directed evolution approach, a mutant strain of BL21(DE3) E. coli has been developed that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product.

With Solu BL21™ a major obstacle to effective protein expression in E. coli has been overcome for many mammalian proteins. This significant improvement should enable scientists to make progress in a wide range of applications quicker and far less expensively than in the past.

Use of the Solu BL21™ Competent E. coli follows a simple and straightforward protocol, the product is compatible with all T7 promoter-based expression vectors.