• Organoid Cryopreservation Handbook
    Human bronchial organoid, showing successful infectious disease model after cryopreservation in STEM CELLBANKER. Red: acetylated α-tubulin, green: SARS-CoV-2-N protein, blue: DAPI. Image courtesy of Kazuo Takayama (CiRA, Kyoto University, Japan)

Laboratory Products

Organoid Cryopreservation Handbook

Nov 28 2022

Cryopreservation of Organoids

AMSBIO has published an informative handbook for scientists who need to preserve or archive organoids in a research lab.

CELLBANKER® cryopreservation media

The handbook brings together published paper citations, quotes and data from leading international research groups who are using the CELLBANKER® range of products to cryopreserve their organoids simply and safely. In addition, the handbook includes best practice protocols plus information making it easier to understand the process of freezing organoids and to choose the optimum cryopreservation media for your application.

Proven capable of recreating aspects of organ structure and function in vivo, organoids are organ-like structures that can be prepared by 3D cell culture and differentiation of stem cells or organ progenitors. Organoids are proving increasingly popular because they maintain genetic information, are CRISPR compatible, expand tractable cell line availability, make sharing libraries easier and are proving useful in drug sensitivity assays. Consequently, use of organoid culture models is now widespread in areas including organogenesis models, drug testing, tumour / disease / infection models, toxicity screening as well as personalised & regenerative medicine.

Validated for cryopreservation and recovery of whole organoids

The CELLBANKER® series of easy-to-use cell cryopreservation media offer superior protection against cell stress during freeze/thaw cycles and have been validated for cryopreservation of organoids. As a cell freezing medium, CELLBANKER® does not require a gradual temperature decrease in a programmed freezer or storage in liquid nitrogen to guarantee efficient cell storage. This makes CELLBANKER® more affordable and accessible than other cell freezing media.

STEM CELLBANKER® is a GMP-compliant, chemically defined freezing medium with formulation optimised for storage of stem and iPS cells and organoids. Supplied ready-to-use with a simple usage protocol, STEM CELLBANKER is completely free of serum and animal derived components and contains only Pharmacopoeia graded ingredients. STEM CELLBANKER significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage.

Download the Organoid Cryopreservation Handbook now.

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