• Antibodies for Exosome Detection
    EC-purified MCF-7-derived exosomes were stained with ExoBrite™ CD9 560/585 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the PE channel.

Laboratory Products

Antibodies for Exosome Detection

Jan 09 2023

ExoBrite™ Antibody Conjugates from Biotium are curated specifically for optimal detection of exosome markers CD9, CD63, and CD81 in isolated exosomes or exosome extracts with little to no background. ExoBrite™ Antibody Conjugates are available in formulations for both flow cytometry and western blotting. 

ExoBrite™ Flow Antibodies are designed for detection of isolated or bead-bound EVs by flow cytometry. The antibodies are available in 4 conjugates: ExoBrite™ 410/450 (Pacific Blue™ channel), ExoBrite™ 490/515 (FITC channel), ExoBrite™ 560/585 (PE channel with yellow laser), and ExoBrite™ R-PE (PE channel). Isotype controls are also available for each conjugate.

ExoBrite™ Western Antibodies are designed for detection of exosome markers in EV extracts by near-IR fluorescent western blot. The antibodies are available with two near-infrared fluorescent dyes, ExoBrite™ 680/700 and ExoBrite™ 770/800, which offer greater signal-to-noise for western blotting than dyes with visible light emission. An ExoBrite™ Western Antibody for detection of calnexin, a membrane protein localised in the endoplasmic reticulum that is not found in exosomes, is also available as a negative control for assessing purity of exosome extracts.

The release of ExoBrite™ Antibody Conjugates add to Biotium’s growing line of optimised solutions for exosome and EV research. Biotium also offers ExoBrite™ EV Membrane Staining Kits which are designed for bright and specific staining of isolated EVs with little to no background. ExoBrite™ EV Membrane Staining Kits are available with four colour options for the Pacific Blue™, FITC, PE, and APC channels.

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