• Antibodies for Exosome Detection
    EC-purified MCF-7-derived exosomes were stained with ExoBrite™ CD9 560/585 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the PE channel.

Laboratory Products

Antibodies for Exosome Detection

Jan 09 2023

ExoBrite™ Antibody Conjugates from Biotium are curated specifically for optimal detection of exosome markers CD9, CD63, and CD81 in isolated exosomes or exosome extracts with little to no background. ExoBrite™ Antibody Conjugates are available in formulations for both flow cytometry and western blotting. 

ExoBrite™ Flow Antibodies are designed for detection of isolated or bead-bound EVs by flow cytometry. The antibodies are available in 4 conjugates: ExoBrite™ 410/450 (Pacific Blue™ channel), ExoBrite™ 490/515 (FITC channel), ExoBrite™ 560/585 (PE channel with yellow laser), and ExoBrite™ R-PE (PE channel). Isotype controls are also available for each conjugate.

ExoBrite™ Western Antibodies are designed for detection of exosome markers in EV extracts by near-IR fluorescent western blot. The antibodies are available with two near-infrared fluorescent dyes, ExoBrite™ 680/700 and ExoBrite™ 770/800, which offer greater signal-to-noise for western blotting than dyes with visible light emission. An ExoBrite™ Western Antibody for detection of calnexin, a membrane protein localised in the endoplasmic reticulum that is not found in exosomes, is also available as a negative control for assessing purity of exosome extracts.

The release of ExoBrite™ Antibody Conjugates add to Biotium’s growing line of optimised solutions for exosome and EV research. Biotium also offers ExoBrite™ EV Membrane Staining Kits which are designed for bright and specific staining of isolated EVs with little to no background. ExoBrite™ EV Membrane Staining Kits are available with four colour options for the Pacific Blue™, FITC, PE, and APC channels.

More information online

Digital Edition

MAC Guide 2023/24

December 2023

Buyers Guide - Major producers of analytical equipment who wish to introduce and sell their products to buyers in science and industry - Over 1,000 products and services that these companys man...

View all digital editions


Instrumentation Live

Jan 17 2024 Birmingham, UK

Smart Factory Expo 2023

Jan 24 2024 Tokyo, Japan

Arab Health

Jan 29 2024 Dubai, UAE

Nano Tech 2024

Jan 31 2024 Tokyo, Japan

SLAS 2024

Feb 03 2024 Boston, MA, USA

View all events