Lysis and Hypotonic Sonication Buffers for ChIP Assays
Dec 08 2022
Offering complete disruption of cytoplasmic and nuclear membranes, Porvair Sciences Chromatrap® buffer chemistries are optimised for sonication and enzymatic shearing.
The success of a ChIP assay is highly dependent on the quality of chromatin prepared. Chromatrap® lysis and hypotonic buffers are high performance reagents you can trust in this critical first step in isolating chromatin from your sample. For enzymatic shearing from fixed cells or tissue - both these detergent-based buffers are ideal for effective fragmentation of nuclear material.
For the disruption of cell membranes, Porvair Sciences optimised Chromatrap® hypotonic buffer composition ensures effective lysis of cell membranes to aid in the release of nuclei material from a wide range of varied species.
Formulated for difficult to lyse cell types and tissues, Porvair Sciences optimised Chromatrap® lysis buffer is proven to enable researchers to better explore and understand the underlying processes and mechanisms that drive biological organisms.
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Lab Asia 30.2 - April 2023
In This Edition Chromatography Articles - On-Column Sample Focussing: a Personal Perspective Mass Spectrometry & Spectroscopy Articles - Multi-scale, multi-modal and operando imaging wi...
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