The Agilent 7890A-5975C GC/MS and
1200SL-6410 Triple Quadrupole LC/MS are used
to analyse melamine and cyanuric acid in pet
food and related raw material. The GC/MS
method employs extraction and then
trimethylsilyl derivatisation and is used here
for screening. The detection limit is 10 μg/g.
The LC/MS/MS method requires only extraction
and then a simple isocratic elution on a
normal-phase silica column (Rx-Sil) with water
and acetonitrile mobile phase containing
5 mM ammonium acetate. The separation
of the two compounds takes 5 min. The
linear range is from 50 pg/mL to 50 ng/mL
for melamine and from 1 ng/mL to 100 ng/mL
for cyanuric acid. The LC/MS/MS method is
demonstrated by analysis of the target
compounds in complex food matrix.
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