Chromatography
LC-NMR – Who in Their Right Mind Would Even Consider it?
Mar 26 2010
Author: Dr Nicolas Haroune
Bernie Monaghan (BM): Could you tell us a little about the group in which you work in Sunderland?
Nicolas Haroune (NH): Chemispec was established in 2000 when the University obtained a grant from the ERDF (European Research Development Fund) to purchase analytical equipment with the aim of helping small and medium enterprises in the North East of England. 2 NMR spectrometers (500 and 300MHz), 1 mass spectrometer (Apex II FT/MS) were purchased. More particularly, the 500MHz spectrometer was equipped with a flow probe for LC-NMR applications. We have now been offering LC-NMR analysis services nationwide for nearly 10 years to industries in the pharmaceutical and chemical sector. We have now been offering LC-NMR analysis services nationwide for nearly 10 years to industries in the pharmaceutical and chemical sector.
BM: Which manufacturers Instruments do you use currently? Are there any technological reasons for making these choices?
NH: These instruments were purchased from Bruker Ltd, a market leading manufacturer in this area.
BM: Can you give the readers some background into the interest and need for such a technique? What are the practical operating issues that have to be overcome to obtain high quality data?
NH: Our LC-NMR equipment allows the detection of only microgrammes quantities of unknowns. It also allows:
• The detection of all protons containing analytes
• It is a non invasive technique so it is possible to recover the sample after analysis
• It provides detailed structural information which are complementary to mass spectrometry analyses
• It represents a time saving since there is no need to collect fraction and as such the sample preparation minimised
These advantages make the LC-NMR suitable for the analysis of components in complex mixture at low concentrations, where difficult to purify or to unstable for isolation by Silica gel chromatography or preparative HPLC.
Digital Edition
Lab Asia 31.2 April 2024
April 2024
In This Edition Chromatography Articles - Approaches to troubleshooting an SPE method for the analysis of oligonucleotides (pt i) - High-precision liquid flow processes demand full fluidic c...
View all digital editions
Events
May 05 2024 Seville, Spain
InformEx Zone at CPhl North America
May 07 2024 Pennsylvania, PA, USA
May 14 2024 Oklahoma City, OK, USA
May 15 2024 Birmingham, UK
May 21 2024 Lagos, Nigeria