Separations scientists are facing an increasing
number of HPLC Columns that are offering either
orthogonal selectivity or extremely high plate
numbers (efficiency) compared to those available
say, 5 years ago. When faced with new
separations that need to be performed, and in
many cases these are getting more complex,
which route do they opt to pursue at the
method development stage?
Much recent commercial activity has seen the
promotion of ultra high efficiency columns,
either by virtue of small particle sizes or use of
elevated temperature (or both) as an option but
we also have some unique novel chemistries,
which are designed to offer alternate selectivity
to the ‘traditional’ end-capped C18 (L1
designation in the US Pharmacopoeia).
In this two-part article we shall examine
the advantages of each route along with
the shortfalls and show examples of
successful separations.
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