Collaboration on Genotyping Arrays for guaranteed sustainability of Wheat Production Announced

Affymetrix, Inc and University of Bristol, Bristol, UK, have announced that they have collaboratively designed a wheat genotyping array and analysed data from experiments as part of an effort to understand wheat genetics and breeding with a goal of ensuring guaranteed sustainable production.

Axiom^® Wheat Genotyping Array (Axiom Wheat Array), consisting of 817,000 markers, was designed for the University of Bristol as part of the Biotechnology and Biological Sciences Research Council (BBSRC)-funded Wheat Improvement Strategic Program (WISP). The aim of WISP is to produce new and novel wheat germplasm via three complementary ‘pillars’ (landraces, synthetics, and ancestral gene introgression), each of which will broaden the pool of genetic variation in elite wheat cultivars by a different route. The design of Axiom Wheat Array is unique because it includes functional and positional annotation information for a variety of wheat species. The SNPs on Axiom Wheat Array include markers from hexaploid landraces and those from the synthetic and ancestral lines exhibiting different ploidy levels.

“The results from Axiom Wheat Array are fantastic and have helped WISP achieve one of the main goals, which is to track the different segments of the 'wheat relative' line and incorporate them into hexaploid bread wheat through the breeding process.  The follow-on focused arrays will offer wheat breeders the capability to understand polymorphisms between their current breeding lines,” stated Professor Keith Edwards, project leader and Professor of Cereal Functional Genomics at the School of Biological Sciences and member of the Cabot Institute, University of Bristol.

A subset of the 817,000 markers found on Axiom Wheat Array will be transferred with 100 percent fidelity onto Axiom 384HT-format arrays to create multiple focused wheat array designs. Each of these Axiom wheat array designs will contain approximately 35,000 markers and each design is expected to serve a different purpose in wheat breeding. The 384HT format enables ultra high-throughput processing of 384 samples simultaneously and will be used to screen thousands of samples in a highly cost-effective manner. Affymetrix will make these new Axiom arrays publicly available before the spring wheat season.