Highly Efficient Assay Kit

A new assay kit for the detection of protein interactions by co-immunoprecipitation is now available from Dualsystems Biotech AG. Formulated to enable highly efficient antigen immunoprecipitation, the FleX-IP immunoprecipitation kit enables immunoprecipitation using less than 10 μg of antibody and is compatible for use with mouse, rabbit, goat, and human antibodies. Typical applications include the validation of protein interactions by co-immunoprecipitation and the immunoprecipitation of proteins from cells and cell-free reactions.

Designed as a complete solution for immunoprecipitation experiments, the FleX-IP kit includes protein A/G agarose, control mouse IgG, lysis/wash buffer, stock NaCl solution, and SDS-PAGE buffer as well as spin columns and collection tubes. Each kit contains sufficient materials for 40 IP reactions and is optimally suited to confirm protein-protein interactions found in DUALhybrid, DUALmembrane, and DUALhunter screens. FleX-IP immunoprecipitations are performed using common IP procedures. The specific antibody is added to the sample to form an immune complex, which is then added to the protein A/G agarose. The complex is washed to remove unbound material and the bound material eluted for downstream applications such as Western blotting.