Enhancing NanoLC for Proteomics

Eksigent, has introduced the cHiPLC-nanoflex System, delivering improved reproducibility and ease of use to proteomics research. The system is a docking station for up to three microfluidic chips that contain nanoLC or trap columns. Eksigent’s proprietary EksPortTM microconnector system ensures reliable and consistent fluidic connections to facilitate column changes. Chips can be interchanged in seconds, even by inexperienced users, with minimal dead volume to compromise resolution. The proprietary microfabrication process used for both the trap chips and analytical column chips delivers exceptional column-to-column reproducibility.

Superior-quality separations and reproducibility are critical to the success of nanoLC-MS-based biomarker validation studies. In these applications, where peptides are measured by multiple reaction monitoring (MRM), the improved column-to-column reproducibility delivered by the cHiPLC-nanoflex helps in obtaining consistent data from large numbers of samples.

The cHiPLC-nanoflex can be added to any of Eksigent’s nanoLC systems. It can also be used with Applied Biosystems Tempo* nano LC systems. cHiPLC-nanoflex and Eksigent’s nanoLC systems can be integrated with most leading mass spectrometers including Thermo, Applied Biosystems/MDS SCIEX and Bruker Daltonics. A built-in 10-port nano valve allows for easy switching between different types of experiments such as direct injection and trap loading.

“Eksigent’s nanoLC systems have already been widely adopted for proteomics LC/MS analysis,” explained Remco van Soest, Vice President of Product Management for Eksigent. “Our new cHiPLC-nanoflex takes the platform to a new level, allowing more users to take advantage of our nano technology.”