Accelerating Sample Preparation

Porvair Sciences Ltd has produced an application note that describes a method for the rapid and sensitive determination of a novel drug entity in human plasma. Key to the high throughput of this method is the use of the Microlute™ 96-well SPE system, which allows a batch of samples to be prepared for injection in less than half the time that is actually required to analyse them. The method meets all regulatory requirements for clinical studies, and is suitable for the analysis of large numbers of samples. A copy of Application Note D may be downloaded from the Porvair website.

The developed method using the Microlute™ was validated over a concentration range of 100 to 10,000 pg /ml. Recovery using the SPE method was approximately 60%, and consistent across the calibration range. The authors describe how the Microlute™ SPE method, unlike protein precipitation sample preparation, is free from LC/MS matrix effects that may influence the ionisation of the compound(s) of interest.

Developed in conjunction with a leading pharmaceutical company the Microlute™ has become the benchmark for high throughput sample preparation across a range of applications. The innovative design of the Microlute™ from Porvair Sciences Ltd offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format capable of rapidly processing 96 samples in one go repeatedly and precisely.