QPCR Optimisation and Troubleshooting

Thermo Fisher Scientific, Inc has launched a new educational tool -- the Thermo Scientific QPCR Optimisation & Troubleshooting Guide. Whether you are beginning to develop a QPCR assay, have a QPCR assay you want to optimise or are getting inconsistent results and don’t know why, this quick reference guide is sure to be a useful resource.

Simply bringing together all the necessary components for QPCR is often not enough to obtain accurate and consistent results. Following a protocol that instructs you how to combine your template with reagents before running them on your chosen instrument only covers the basics of real-time PCR. This guide aims to educate researchers that are new to the technique to better understand the science of real-time PCR. It should enable them to develop their skills in troubleshooting and optimisation. The Thermo Scientific QPCR Optimisation & Troubleshooting Guide provides a quick reference to help you achieve the best results possible on a consistent basis. The Optimisation section of the guide covers parameters such as choice of plate, amplicon size, primer design, and it makes recommendations to optimise QPCR performance. Whilst in the troubleshooting section, problems, possible causes and remedial actions are addressed.

Whether users are an ‘old hand’ or just starting out in their laboratory career, the Guide is sure to be of value. It brings together the knowledge that Thermo Fisher Scientific

has accumulated during the 20 years the company has manufactured and optimised PCR reagents and plastics for the life science industry.