Kit for Genome-wide Profiling of DNA-Protein Interactions

EMD Millipore announced the launch of the Magna ChIP-Seq preparation kit designed to simplify genome-wide profiling of DNAprotein interactions by chromatin immunoprecipitation and next generation sequencing (ChIP-Seq). “Genome-wide mapping of DNA-protein interactions using ChIP-Seq is indispensible for many gene regulation studies but the techniques can be challenging,” noted John Rosenfeld, PhD, Manager, Chromatin Biology Group, EMD Millipore. “With the all-in-one Magna ChIP-Seq kit, this process is now simplified, enabling both experienced and new labs to more effectively leverage this powerful technique.”

In contrast to other products that support only part of the ChIP-Seq workflow, the Magna ChIP-Seq kit is a complete solution that provides a set of validated reagents for both chromatin immunoprecipitation and next generation library construction. These reagents, in combination with a detailed protocol and quality control guidelines, simplify the ChIP-Seq process to allow researchers to map interactions of histones, transcription factors, and other chromatin associated proteins on a genome-wide scale.

As successful immunoprecipitation is critical to construction of the sequencing library, the kit includes positive and negative control antibodies and a set of PCR primers that can be used as in-process controls or to verify technique. The kit has been used to construct ChIP-Seq libraries from as little as 1 ng of purified ChIP DNA, meaning that DNA segments associated with low abundance proteins can be sequenced.