Obtaining Sharper Clearer Images

The Olympus cellSens deconvolution solution module employs a constrained iterative (CI) algorithm to remove out-of-focus blur in advanced life science imaging. This high-speed operation provides extremely sharp images, as well as near confocal quality, with the ability to correct for any spherical aberrations. Integrating seamlessly with the cellSens Dimensions software, outstanding performance and quality are guaranteed.

Using a specialised and improved algorithm developed exclusively for Olympus, the deconvolution solution module provides advanced, high-speed image restoration. Maximising modern PC computing capabilities, it leverages multi-core processor architectures to provide an extremely powerful deconvolution facility, enabling the sharpening of even the most blurred images. Consequently, users can obtain a fast and easy-to-use, comprehensive deconvolution, removing the complex parameter adjustment steps required with other advanced de-blurring algorithms.

The resulting high-quality image stacks can be visualised in three dimensions, using the powerful VoxelViewer to display structures and ISO projections via a simple click of the mouse. This enables more in-depth analysis, with the capability to display stereo adaptations of the image. Furthermore, the online de-blur functionality enables users to visualise the deconvoluted images during the live operation of the camera.

A broad range of image types can be deconvolved using the Olympus deconvolution solution module, including fluorescence, confocal and brightfield. With the additional capability to perform blind deconvolution, the software is able to use a theoretical point spread function (PSF), adapting it to the specific data. Furthermore, the PSF calculation can be saved and applied when performing subsequent deconvolutions. In addition, images obtained from non-Olympus systems are also easily imported, making it a highly versatile imaging solution.

Designed to offer control and processing for microscope-based experimental systems, the cellSens Dimensions software package provides a broad range of advanced features, as well as specialised, optional solution modules.