Microscopy & Microtechniques
Cell Culture Flask with Removable Microscope Slide Provides Superior Measurements for Fluorescence Applications
Jan 09 2018 Read 870 Times
Clipmax is a 10cm2 tissue flask with a standard microscope slide (25mm x 75 mm) as a growing surface. It is intended for cell culture, staining, and immunofluorescence as it does not interfere with fluorescent labels-directly on the slide. It is also useful for viral and mycoplasma testing, chromosome studies toxicity tests and immune cytology. The growth area of the slide is opto-mechanically treated for an optimal cell adhesion and proliferation.
After the cell culture the medium chamber with the biocompatible seal ring can easily be detached from the slide. Cells may then be fixed, stained; cover slipped, and filed with the usual convenience and can be used directly for microscopic view. After removing the chamber of the Clipmax, the microscope slide with the cells can be fixed and stained directly on the microscope slide and can be used directly for immunocytochemistry and for various cell analyses. The transmission of the crystal-clear microscope slide is reported to be possible as of 250nm with a tailing at 280-300nm and stable between 350–400nm until plus 750nm (+92% transmission within 400-800nm). The fluoroscopic measurements are better than with glass, PC, or PS and the protein absorption is less than using glass slides. The thermal and optical features of the Clipmax slide are better than those made from PC, PET or TAC and in addition the slide is resistant to numerous chemicals such as Xylolite, alcohol, ether, (HCl, HNO3, H2SO4), (NaOH, NH4OH) or even H2O2. Also, biological coatings, i.g. with collagen, result in no loss of measuring signals. Finally, the handling of the Clipmax slide is much easier because there is no risk of injury, as it is plastic and not glass.
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