Fluorescence and circular dichroism (CD) spectroscopy are well represented in the literature of macromolecular studies.
Polarisation of fluorescence (POF) and Circularly Polarised Luminescence (CPL) are less utilised highly sensitive probes of molecular interactions (e.g. protein folding, secondary structure formation).
Whereas CD involves the ground state of a chiral molecule, CPL is emitted by a fluorophore if the excited state of the emitting species is chiral. Fluorescence offers insights into the environment surrounding the emitting molecule; anisotropy (POF) is sensitive to rotational motion of the fluorescent probe.
These four probes provide complementary information, with POF and CPL providing insights into the structure, interactions, and environment of the emitting molecule.
A recently introduced Olis upgrade accessory adds both CPL and POF to any Olis CD or fluorescence spectrometer for kinetic or steady-state readings. Single purpose models are also available.
Product directory
