Laboratory Products

  • New Tissue Clearing Kits and Reagents for Faster, Accurate 3D Imaging
    Albumin antibody ab207327 (yellow), CD68 antibody (green), Vimentin antibody (red), and DAPI (blue) used with 3D Cell Culture Clearing Kit ab243299 to clear and stain a 3D hepatocyte cell culture spheroid.
  • NeuN antibody ab177487 (green) and DAPI (blue) used with Tissue Clearing Kit ab243298 to clear and stain a 1 mm thick coronal section of mouse brain.

New Tissue Clearing Kits and Reagents for Faster, Accurate 3D Imaging

May 23 2019 Read 293 Times

Abcam’s tissue clearing kits and reagents enable easy and rapid clearing for 3D imaging of tissues and cell culture models.

Traditionally, 3D imaging of thick tissue sections and 3D cell culture models (organoids, microtissues, spheroids) has presented many technical challenges, including laborious and time-consuming tissue processing, layer-by-layer 2D imaging of thin sections and in silico 3D reconstruction. By contrast, tissue clearing reagents can expedite detailed 3D imaging by reducing the need for these steps, while providing a truer reflection of biological structures.

Developed with Visikol’s HISTO™ technology, Abcam’s tissue clearing kits use simple protocols, standard laboratory equipment, and are compatible with immunostaining, fluorescent protein and chemical dyes. Clearing is also reversible, for conventional H&E or IHC staining following 3D imaging.

To facilitate the set-up and use of Abcam’s tissue clearing kits, a selection of recombinant antibodies for key markers (Neun, S100 and Iba1) have been validated for use with the kits. Tissue clearing reagents can be purchased as part of a kit or as individual products, for greater flexibility.

For further information please click here.

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment




Digital Edition

International Labmate Buyers' Guide 2019

June 2019

In This Editon Articles - More than One Way to Hit the Bullseye - Gas Chromatography - Vacuum Ultraviolet Spectroscopy - News Bites from the UK Laboratory Industry Mass Spectrometry & Sp...

View all digital editions

Events

Chemspec Europe

Jun 26 2019 Basel, Switzerland

MMC 2019

Jul 01 2019 Manchester, UK

INTERPHEX JAPAN 2019

Jul 03 2019 Tokyo, Japan

PREP 2019

Jul 07 2019 Baltimore, MD, USA

analytica Lab Africa

Jul 09 2019 Johannesburg, South Africa

View all events