Kit To Assist in Detection of Endogenous Peptides

Denator AB announced that it has launched the first in a series of kits designed for use with the company’s

heat-stabilisation system. The Stabilizor™ Peptide Extraction Kit enables users to heat-stabilise, homogenise

and extract endogenous peptides from tissue samples prior to analysis by liquid chromatography-mass

spectrometry (LC-MS).

During downstream analysis, the presence of endogenous peptides, often existing at very low levels in vivo, can

be masked by peptide fragments generated from normal degradation processes. Utilisation of Denator’s Stabilizor

system, based on the company’s heat-stabilisation technology, has been shown to stop tissue degradation from

the moment of sampling, preventing the enzymatic processes that generate peptide fragments.

This news will be of particular interest to researchers looking at the role of neuropeptides in neurogenerative

diseases. A recently published paper demonstrated an increased recovery of neuropeptides from tissue samples

when utilising heat stabilisation followed by an optimal extraction protocol (Neuropeptide profiling of the

bovine hypothalamus: Thermal stabilisation is an effective tool in inhibiting post-mortem degradation.

Olof Sköld, CEO at Denator, explained further: “Working closely with users of our system has revealed that the

quality of final results can be influenced by the extraction methods used after heat stabilisation.

This new kit offers the convenience of having all necessary accessories and solutions ready to use, and, most

importantly, includes a validated protocol that aims to maximise the recovery of endogenous, water-soluble

peptides for subsequent analysis by LC-MS. We see this development as a natural step to ensure that

researchers who heat-stabilise tissues upstream achieve the best possible analytical results downstream.”

Implementation of the Stabilizor solution in laboratory workflows is reflected in a growing number of peerreviewed

publications particularly from proteomic laboratories. Scientists are able to perform many analyses

without risk of sample degradation, confident that their results truly reflect the in vivo situation.