Innovative 2D-Gradient for Enhanced PCR Optimisation

Optimising the annealing temperature to improve PCR results is a routine task, so why not optimise as far as you can? More and more findings indicate that optimisation of the denaturation temperature is worthwhile as well. A high denaturation temperature will harm the enzyme and other biomolecules in your assay. A low denaturation temperature may result in inefficient splitting of the complimentary DNA strands, as is often found with GC-rich templates or templates prone to form ‘hairpin structures’.

Eppendorf’s new 2D-Gradient allows optimisation of the annealing and the denaturation temperature in a single run – taking less time than ever before. This gives you the ideal set of temperatures for your PCR assay - quickly, conveniently, reliably.

Read the application note.

More information online