• Elispot Software Speeds Up Vaccine Research

Microscopy & Microtechniques

Elispot Software Speeds Up Vaccine Research

Apr 25 2008

Software designed to speed up tumour, AIDS and vaccine research is being released by Carl Zeiss. The new KS Elispot application combines with various Zeiss microscope systems to make Elispot reading faster, more sensitive and more convenient for immunologists, oncologists and pharmacologists and also enables fluorescence Elispot evaluation.
The KS Elispot compact based around the Zeiss Stemi 2000 C stereomicroscopes will read and evaluate the results of a 96-well microplate in less than four minutes and is ideally suited to the routine laboratory. For the research laboratory, the Axio Imager based KS Elispot will distinguish spots as small as two microns and, combined with the AxioCam camera and suitable filters, enables fluorescence Elispot evaluation.
Whether in normal illumination or fluorescence mode, the new software
allows users to set up convenient shortcuts to established operating
parameters rather than searching through a series of drop-down menus. For instance, opening the configuration panel and setting the exposure time of the camera is a single mouse click. A neutral frame can be added to single well images to improve spot recognition at the
periphery. Errors are also reduced with a facility that stops the AxioCam camera imaging in the reduced-resolution binning mode
and re-evaluation of stored images can also be blocked to prevent unintentional or unauthorised changes. Finally, KS Elispot software has been designed to integrate seamlessly with the Zeiss imaging suite, AxioVision 4.6. Elispot (Enzyme-linked immunosorbent spot) is a highly sensitive version of the Elisa immunoassay used routinely for monitoring immune responses in humans and animals.
Originally developed to enumerate B cells secreting antigen-specific antibodies, Elispot has been adapted for various tasks, especially the identification and enumeration of cytokineproducing cells at the single cell level where they allow visualisation of the secretory product of individual activated or responding cells. Because each spot represents a single reactive cell, Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells)information. Limits of detection are below 1/100,000 rendering the assay uniquely useful for monitoring antigen-specific responses, applicable to a wide range of areas of immunology research, including cancer, transplantation, infectious disease, and vaccine development.

Digital Edition

Lab Asia 31.2 April 2024

April 2024

In This Edition Chromatography Articles - Approaches to troubleshooting an SPE method for the analysis of oligonucleotides (pt i) - High-precision liquid flow processes demand full fluidic c...

View all digital editions

Events

Microbiology Society Annual Conference 2024

Apr 08 2024 Edinburgh 2024

analytica 2024

Apr 09 2024 Munich, Germany

ChemBio Finland 2024

Apr 10 2024 Helsinki, Finland

Analytica Anacon India & IndiaLabExpo

Apr 15 2024 Mumbai, India

Analitika Expo 2024

Apr 16 2024 Moscow, Russia

View all events