• Brighter Fluorescence and TIRF Imaging; Lost Light Recaptured

Microscopy & Microtechniques

Brighter Fluorescence and TIRF Imaging; Lost Light Recaptured

Jan 29 2008

High resolution phase contrast and fluorescence imaging is now possible using the same objective, thanks to the unique external phase contrast system on Nikon Instruments' new Ti Series of inverted microscopes.

Furthermore this flexibility is now achieved with maximum light transmission. Available on all models, the external unit locates the phase ring within the main microscope body thus allowing for the use of high NA and TIRF objectives, for the acquisition of high quality phase contrast images.

The Ti Series exclusive use of high NA objectives without phase rings to achieve phase contrast, enables the capture of brighter fluorescence images; essentially recapturing the 10-15% of light traditionally blocked out by the phase annulus in the objective. In addition, the external phase contrast unit minimises both aberration and double image acquisition to achieve crisp definition of cells.

Digital Edition

ILM 49.5 July

July 2024

Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...

View all digital editions

Events

ADLM 2024

Jul 28 2024 San Diego, CA USA

InaLab 2024

Jul 30 2024 Jakarta, Indonesia

Miconex

Jul 31 2024 Chengdu, China

ACS National Meeting - Fall 2024

Aug 18 2024 Denver, CO, USA

EMC2024

Aug 25 2024 Copenhagen, Denmark

View all events