• Brighter Fluorescence and TIRF Imaging; Lost Light Recaptured

Microscopy & Microtechniques

Brighter Fluorescence and TIRF Imaging; Lost Light Recaptured

Jan 29 2008

High resolution phase contrast and fluorescence imaging is now possible using the same objective, thanks to the unique external phase contrast system on Nikon Instruments' new Ti Series of inverted microscopes.

Furthermore this flexibility is now achieved with maximum light transmission. Available on all models, the external unit locates the phase ring within the main microscope body thus allowing for the use of high NA and TIRF objectives, for the acquisition of high quality phase contrast images.

The Ti Series exclusive use of high NA objectives without phase rings to achieve phase contrast, enables the capture of brighter fluorescence images; essentially recapturing the 10-15% of light traditionally blocked out by the phase annulus in the objective. In addition, the external phase contrast unit minimises both aberration and double image acquisition to achieve crisp definition of cells.

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