Revolutionised sample preparation for biological samples

The MonoSpin series from GL Sciences has been optimised for use as a spin column in the sample preparation of biological samples, such as blood, serum and urine. It is an innovative tool utilising silica monoliths as its base material. Silica monoliths possess uniform continuous pores, facilitating rapid separation and high-sensitivity analysis. Utilising short-time centrifugation for solid-phase extraction, the entire sample treatment process can be completed within ten minutes.

The MonoSpin series offers cartridges in different types: the Spin Type, ideal for pretreating samples ranging from 50 to 800µL; the Large Type, suitable for samples ranging from 0.5 to 8mL; and the 96-well Plate Type, available for multi-analyte analysis with the same spin column volume.

Disk-shaped silica monoliths do not require frits to hold particle media, as in traditional solid-phase extraction cartridges. Monolithic material provides a substantial surface area, allowing for sample volume reduction. Silica monoliths retain samples within the cartridge and facilitate complete elution of small samples during processing. Despite its high liquid permeability, it allows for rapid elution without compromising high recovery, owing to swift sample diffusion and separation.

The series can be used for desalting, purification and fractionation of peptide samples; protein recovery and purification; purification post iTRAQ derivatisation; purification of glycans; recovery of drugs from biological samples (urine, serum, plasma); purification of catecholamines; and recovery and purification of organic acids.

Due to its high permeability, the MonoSpin series enables quicker and more efficient purification and enrichment through centrifugation. It is also recommended for eluting small-volume samples, allowing for the collection of trace analytes without dilution.