Real-time Biochemistry of Living Cells and Whole Organisms
May 31 2019 Read 257 Times
Amsbio has added a new range of GCaMP calcium indicators (jGCaMP7b, jGCaMP7c, jGCaMP7f, jGCaMP7s) to their portfolio of ready-to-use adeno-associated virus (AAV) biosensor products. These viruses encoded biosensors are supplied ready for use for neural activity studies in vivo.
AAV Biosensors provide a snapshot of intracellular interactions, specifically active brain circuitry. Using fluorescent protein-based AAV biosensors allows permanent marking of active cells over short time scales, providing a window into the real-time biochemistry of living cells and whole organisms.
GCaMP is a genetically encoded calcium indicator (GECI) generated from a fusion of the green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. Upon binding of GECI to Ca2+ there is an induction of a change in fluorescence signal. It is this change in signal that allows for measurement of the action potentials, and other receptor activation events, that trigger Ca2+ fluxes. GCaMP7 are the 7th generation of GCaMP, offering improved engineering for increased signal-to-noise ratio and much faster kinetics, when compared to previous versions, specifically, GCaMP3 and GCaMP5G.
Amsbio AAV biosensors are introduced to cells, tissues or organisms to detect changes by fluorescent microscopy or spectrum changes. These biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, these biosensors permit signalling pathway exploration or allow the measurement of a biomolecule. They do all this while preserving both spatial and temporal cellular processes.
Amsbio offers a range of Calcium or Glutamate Biosensor products that come ready-to-use, with a choice of promoter and the ability to include the Cre inducible (FLEx-ON) expression. Amsbio has packaged these indicators into the most commonly used AAV serotypes (AAV8 and AAV9). Different serotypes are available on request.
Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.
In This Edition Chromatography - Vacuum Ultraviolet Spectroscopy: A Versatile Tool for Analysis of Gasoline and Jet Fuels - High-performance Columns and Bulk for your Chromatography - New L...
View all digital editions
Oct 22 2019 Cologne, Germany
Oct 22 2019 New York, USA
Oct 23 2019 Beijing, China
Oct 30 2019 Birmingham, UK
Nov 03 2019 Toronto, Canada