High Throughput Screening of Cellular Bioenergetics

Integra has published a video demonstrating how its VIAFLO 96 handheld electronic pipette, used in conjunction with the Seahorse Bioscience XF96 Extracellular Flux Analyser, provides high throughput screening of the cellular bioenergetics of multiple cell lines.

The measurement of cellular bioenergetics, the processes by which cells produce and consume energy, is fundamental to determining the growth, development, function and metabolism of cells. The Seahorse Bioscience XF96 Extracellular Flux Analyser enables the determination of the two major energy yielding pathways in cells, aerobic respiration and glycolysis, by simultaneously measuring the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR).

In laboratory tests the VIAFLO 96 is shown to simplify and significantly accelerate the loading of substrates, inhibitors and stimulators into the XF96 Extracellular Flux Assay Cartridges prior to making cellular bioenergetics measurements.

The VIAFLO 96 from Integra is an internationally proven 96-channel handheld electronic pipette that enables fast, precise and easy simultaneous transfer of 96 samples. The system has been designed such that 96 channel pipetting is as easy as single channel pipetting, consequently users are able to immediately benefit from the increased productivity delivered by their VIAFLO 96.

 Using an XF96 Extracellular Flux Analyser, investigators can measure the effects of up to four compounds on cellular metabolism, as well as determine the basal oxygen consumption and glycolysis rates.  The XF96 Extracellular Flux Analyser can also generate data on respiratory capacity, mitochondrial function, fatty acid oxidation, glycolytic function, fatty acid, and amino acid metabolism, as well as ATP turnover and cell signalling in primary, adherent, tumour and suspension cells, islets, and isolated mitochondria. This cellular information is of considerable importance to researchers developing new drugs for cancer, aging, and metabolic, cardiovascular, and neurodegenerative diseases.