• Direct translocation of proteins into living cells
    Efficient direct translocation of proteins into living cells using BioPORTER® protein delivery reagent. (courtesy: AMSBIO)
  • Primary rat monocytes were loaded with NGF using BioPorter®. NGF (FITC) and Nuclei (DAPI). Image from Böttger et al (2010).

Reagents

Direct translocation of proteins into living cells

BioPORTER® protein delivery reagent from AMSBIO is proven to efficiently transport functionally active proteins directly into living cells eliminating the traditional DNA transfection, transcription, and protein translation process.

Employing a unique lipid-based protein delivery system, BioPORTER® makes studying protein functionality faster and easier than ever before. Comprising a proprietary cationic lipid mixture that interacts non-covalently with the protein, BioPORTER® creates a protective vehicle for immediate protein delivery directly into the cell cytoplasm.

BioPORTER® has been demonstrated to be highly effective in delivering a diverse array of proteins, peptides, and other macromolecules into the cytoplasm of many different cells, retaining the structure of the proteins whilst leaving the transduced cells unharmed. The BioPORTER® Reagent is especially useful when studying protein function in cells that are difficult to transfect using traditional DNA transfection reagents, such as human dendritic cells.

Available either as a bulk reagent or convenient single-use QuikEase™ vials, BioPORTER® is extremely easy to use. For maximum speed and convenience, AMSBIO offers the BioPORTER QuikEase™ Kit. Each QuikEase kit contains 96 single reaction size vials that are pre-coated with BioPORTER® reagent. You can either separate each vial for single use or use the entire pack for high-throughput experiments. The BioPORTER QuikEase kit saves hours of time by eliminating the need to coat the BioPORTER® Reagent onto individual vials.

Read AMSBIO’s blog post to learn more about how BioPORTER® has been used in immune cell driven drug delivery for treating neurodegenerative disorders.

More information online


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