Laboratory Products

  • CLARIOstar Plus - ‘Micro Plate Reader - redefined’
    Figure 1: LVF Monochromator Light Transmission
  • Figure 2: Linear Variable Dichroic Mirror
  • Figure 3: Cross Talk Reduction

CLARIOstar Plus - ‘Micro Plate Reader - redefined’

Jun 11 2019 Read 134 Times

The CLARIOstar® Plus multi-mode microplate reader is equipped with a host of unique features that provide significant benefits for plate-based assays.

It is the only microplate reader to feature Linear Variable Filter (LVF) MonochromatorsTM. These significantly increase light transmission over conventional grating-based monochromators, providing filter-like sensitivity and wavelength flexibility. Whether you are working with new or unique fluorophores and lumiphores, you always have the access to the optimal settings to provide the very best instrument performance for your assay.

Microplate readers use dichroic mirrors to separate the emission from excitation light. The CLARIOstar Plus Linear Variable Dichroic Mirror automatically adjusts to perfectly match the LVF Monochromator excitation and emission settings. This significantly reduces the background signal, thus improving instrument sensitivity by more efficiently separating emission and excitation light.

The new Enhanced Dynamic Range (EDR) technology significantly simplifies measurement setup and provides a much easier solution for assay development, eliminating the need to repeat measurement with different instrument settings. EDR ensures reliable detection of samples over the largest range of concentrations and signal intensities (8 decades) in a single measurement.

The software includes a ‘Fluorophore Toolbox’ with 50 predefined settings for commercially available fluorophores. This can be updated to include new fluorophores by scanning with optimal detection settings automatically determined. Multiple fluorophore spectra can be displayed, allowing easy setup of FRET and BRET assays by drag and drop LVF excitation and emission settings.

In cell based assays, the use of red-shifted fluorophores significantly reduces cell and media based auto-fluorescence. Measurement of red dyes requires an extended wavelength detector to ensure high sensitivity up to 900 nm, ideal for existing red fluorophores such as Alexa 647 and perfect for the ever expanding catalogue of red dyes. With dedicated detectors for fluorescence, luminescence and absorbance the CLARIOstar Plus ensures superior performance in all read modes.

Discrimination between very low and very high luminescence signals can be compromised especially when they are proximal on the microplate. Advanced software and hardware features of the CLARIOstar Plus significantly reduce cross-talk, improving assay performance in AlphaScreen® and luminescence screening in all plate formats including 384 and 1536-well plates.

Absorbance measurement is quick and easy with the UV/vis absorbance spectrometer. Unlike with conventional monochromators, no mechanical movement is required. The spectrometer instantly captures an entire spectrum from 220 to 1000 nm in less than 1 second/well. This speed advantage is perfect for spectral acquisition when looking at nucleic acid purity for example or for measuring spectral shifts like the conversion of haemoglobin to met-haemoglobin.

The CLARIOstar Plus features the very latest and unique technologies in microplate reading with a whole host of benefits and as such represents the must have microplate reader for your lab.

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International Labmate Buyers' Guide 2019

June 2019

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