Microscopy & Microtechniques
New Fluorescent Nucleic Acid Gel Stain is a Highly Sensitive Ethidium Bromide Alternative
Feb 13 2006 Comments 0
GelRed offers an excellent alternative to the widely used ethidium bromide (EB) stain, which is not only highly mutagenic but often requires a destaining step to reduce background fluorescence. GelRed is highly stable so, when used as a precast gel stain, it can be microwaved with agarose or subjected to other heating procedures commonly used in gel preparation. Importantly, gels can also be safely and conveniently stored for later use without compromising performance.
The GelRed dye has a major excitation peak at around 300 nm and a red emission at around 595 nm meaning it can be optimally excited with a standard 300 nm UV transilluminator while its fluorescence emission is completely compatible with a standard EB filter or SYBR filter.
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