iPSC protocol advances research into male infertility treatments

Amsbio highlights recent progress by the Sasaki Lab at the University of Pennsylvania’s School of Veterinary Medicine in developing a novel protocol that uses induced pluripotent stem cells (iPSCs) to create reconstituted human testes.

Since their pioneering 2020 study, Dr Kotaro Sasaki’s team has made significant strides in generating human testis-like tissue from iPSCs. Their latest breakthrough, detailed in a 2025 blog update, introduces a refined method for producing xenogeneic reconstituted testes (xrTestes) capable of supporting human spermatogonial development in vitro.

This advancement reinforces the Sasaki Lab’s system as a powerful and scalable model to study early human spermatogenesis, providing a valuable platform for reproductive biology research. These developments could open new pathways for investigating and developing treatments for male infertility.

Supporting these efforts, Amsbio supplies a comprehensive range of reagents designed for stem cell culture, differentiation, and 3D tissue modelling.

The Sasaki Lab relies on Amsbio’s StemFit® feeder-free, chemically defined media to maintain their iPSC cultures. For coating culture plates, they use recombinant laminin E8 fragments delivered via iMatrix-511 silk from Amsbio. The lab also preserves cells with CELLBANKER 1, a trusted cryopreservation solution for sensitive cell lines. Additionally, CELLOTION™ cell wash and recovery solution is employed for collecting FACS-sorted cells before single-cell RNA sequencing.

To explore the Sasaki Lab’s latest advances in iPSC-derived human testes research, read the full 2025 blog update from Amsbio. 

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