New Hydrophobic Interaction Column Offers additional Selectivity for Separating Monoclonal Antibodies or Antibody-drug Conjugates

Many laboratories analysing monoclonal antibodies (mAb) regularly encounter situations where they need alternative hydrophobic interaction chromatography (HIC) selectivity. Thermo Fisher Scientific has developed new column chemistry that separates many more of these challenging mAbs, antibody-drug conjugates (ADCs) and proteins.

The Thermo Scientific MAbPac HIC-10 HPLC column has been demonstrated to provide selectivity that is different from other commercial HIC columns, including separating many native mAbs, fragments, aggregates, oxidation variants, PEGylated mAbs and ADC.

The column’s proprietary chemistry is designed to provide the new selectivity in a high-resolution column with excellent biocompatibility and high recovery. The column is compatible with organic solvent and aqueous mobile phase. It is designed for rugged stability and very low carry-over.

“MAbs are the most successful and prominent class of biotherapeutics,” said Ilze Birznieks, product line manager, biocolumns, Thermo Fisher Scientific. “HIC is a popular technique for separating these biomolecules, but researchers have been continually asking for alternative selectivity. This new column responds to these customer demands.”