How can gDNA be Quickly Removed from RNA Samples?

DNA / RNA

How can gDNA be Quickly Removed from RNA Samples?

09 Jun, 2021

Published over 5 years ago. See the latest and most current information on DNA / RNA.

Why is it important to remove gDNA from RNA samples?

Removal of gDNA from RNA samples is of particular important to scientists undertaking RT-PCR, RNA-seq and microarray-based applications where the high purity and accurate measurement of RNA is vital.

The newly launched Chromatrap® gDNA Removal Columns from Porvair Sciences rapidly removes unwanted genomic DNA (gDNA) from RNA samples.

•    30 second, enzyme-free gDNA removal

•    Load and spin method for gDNA-free, total RNA

•    Highly specific gDNA removal. Save precious RNA sample

•    Ideal for DNA-sensitive applications 

Employing a simple load and spin method the new columns efficiently capture gDNA on the membrane allowing efficient flow through of DNA-free cell lysates for downstream RNA purification. Unlike traditional gDNA removal methods, Chromatrap® gDNA removal columns require no time-consuming enzymatic digeston that can also impact RNA purity. 

Beneficially using the Chromatrap® spin column methodology the captured gDNA may also be eluted for use in downstream applications such as g PCR, Southern blotting, cloning and sequencing. 

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