Laboratory Products

  • Real Time Cell based Assays in a Microplate Reader
    Figure 1. The CLARIOstar® from BMG LABTECH.
  • Figure 3. Example of ischaemia/reperfusion conditions mimicked in the CLARIOstar® with ACU.
  • Figure 2. Multiplexed RealTime-Glo® and cellToxTM Green assay. Average results of 10 replicates shows that cell viability increases and cytotoxicity is unchanged over 72 hours in untreated cells.

Real Time Cell based Assays in a Microplate Reader

May 21 2018 Read 630 Times

Cell-based assays have become an important application in the life science sector widely used in biotech companies and academic research institutions to monitor cell health in real-time.

Experiments undertaken with living cells require closely regulated physiological conditions including temperature and gas control. All BMG LABTECH microplate readers include precise temperature control as standard and the addition of the Atmospheric Control Unit (ACU) provides independent control of both oxygen and carbon dioxide for cell based assays.

BMG LABTECH’s Atmospheric Control Unit enables the independent control of both oxygen and carbon dioxide concentrations within the plate reader and replaces the requirement for manual intervention required for transferring plates between the incubator and the microplate reader, especially useful for kinetic assays saving time and labour and avoiding any temperature or atmospheric stress to the cells. It is the ideal solution for a wide range of cell bases assays, including long-term cell proliferation, hypoxia and cytotoxicity studies.

Available across the BMG LABTECH microplate reader range the Atmospheric Control Unit is accessible to all researchers whether using the single-mode Omega readers, LUMIstar®, SPECTROstar® and NEPHELOstar®, the multimode FLUOstar® Omega and POLARstar® Omega or the more sensitive and flexible CLARIOstar® range.

The OMEGA multimode readers are perfect instruments for general life science laboratories providing an economic solution for multiplex assays including simultaneous reads of fluorescence, absorbance and luminescence, ideal for measuring reporter gene expression whilst following cell growth and proliferation or cytotoxicity.

(NOTE this needs to be high res and text fig 3 removing)

The more advanced features of the CLARIOstar and its associated Atmospheric Control Unit provides more opportunities to perform more elaborate assays with greater sensitivity.

Exclusive to the CLARIOstar Atmospheric Control Unit are unique features including tracking real-time gas values and gas ramping capabilities. Tracking the gas levels directly in the MARS data analysis software throughout the assay provides retrospective confirmation of the O2 and CO2 levels when analysing data. This provides confirmation that the programmed gas and temperature conditions were maintained throughout and helps relate cells behaviour in relation to these conditions. The programmable controlled gas ramping provides for the first time in a microplate reader the ability to perform ischaemia reperfusion type experiments that can be monitored in real time and related directly to the gas concentrations. Ischaemia reperfusion assays reproduce the O2 changes cells experience with life-threatening conditions such as stroke, myocardial infarction or renal failure, whereby cells are temporarily deprived of O2 and ischaemia - an essential nutrient. In-vitro experiments require an instrument capable of rapid deoxygenation, rapid reperfusion together with the parallel monitoring of critical biological parameters including cellular oxygenation and ROS, currently only possible on the BMG LABTECH CLARIOstar.

The precise control of gases in all the BMG LABTECH microplate readers allows reproducible conditions to be programmed for all assays replicating physiological conditions pertinent to the cell line or tissue that you are studying.

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