How can gDNA be Quickly Removed from RNA Samples?
Jun 09 2021
Why is it important to remove gDNA from RNA samples?
Removal of gDNA from RNA samples is of particular important to scientists undertaking RT-PCR, RNA-seq and microarray-based applications where the high purity and accurate measurement of RNA is vital.
The newly launched Chromatrap® gDNA Removal Columns from Porvair Sciences rapidly removes unwanted genomic DNA (gDNA) from RNA samples.
• 30 second, enzyme-free gDNA removal
• Load and spin method for gDNA-free, total RNA
• Highly specific gDNA removal. Save precious RNA sample
• Ideal for DNA-sensitive applications
Employing a simple load and spin method the new columns efficiently capture gDNA on the membrane allowing efficient flow through of DNA-free cell lysates for downstream RNA purification. Unlike traditional gDNA removal methods, Chromatrap® gDNA removal columns require no time-consuming enzymatic digeston that can also impact RNA purity.
Beneficially using the Chromatrap® spin column methodology the captured gDNA may also be eluted for use in downstream applications such as g PCR, Southern blotting, cloning and sequencing.
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